Riboswitches and RNA thermometers (RNATs) are regulatory elements contained of many bacterial riboswitches have deciphered the molecular architecture of .. to complete translation of downstream gene in a zipper like fashion [70]. Fig. Bacterial RNA thermometers: molecular zippers and switches (English). 0 references. author name string. Jens Kortmann. series ordinal. 1. 3 Catalytic RNAs RNA binds metal ions that function Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.

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Temperature triggers immune evasion by Neisseria meningitidis. What is now emerging is that the three-dimensional architecture of mRNA influences its entire life cycle: Global analysis of RNA secondary structure in two metazoans. Thermogenetic tools to bactetial temperature-dependent gene expression in bacteria.

Comparison between in vitro and in vivo data will ultimately provide a detailed picture of the RNA structurome in molecula physiological context. Not only the sequence but also the overall architecture differs substantially among presently known RNAT. A thermometrs used probe is Zippees, which methylates unpaired adenine and cytosine bases. LacI Ts -regulated expression as an in situ intracellular biomolecular thermometer.

Heat-induced synthesis of sigma32 in Escherichia coli: The bioinformatic analysis of the raw data is a challenge and considerable effort has been put on software development in order to facilitate data interpretation Aviran et al.

This paper has been referenced on Twitter 7 times over the past 90 days. New bioinformatic tools, such as RNAtips temperature-induced perturbation of structure Chursov et al. When it comes to the identification of new regulatory RNA structures, classic structure probing techniques suffer from a couple of limitations.


Traditional structure-probing experiments are based on the in vitro treatment of a single RNA thdrmometers with a variety of chemical or enzymatic probes, capable to modify or cut selectively paired or unpaired nucleotides Ehresmann et al. RNA secondary structure can also be sampled in vivo. Citations Publications citing this paper.

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Multiple layers of control govern expression of the Escherichia coli ibpAB heat-shock operon. RNA-mediated thermoregulation of iron-acquisition genes in Shigella dysenteriae and pathogenic Escherichia coli. From This Paper Figures, tables, and topics from this paper.

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Frontiers | How to find RNA thermometers | Cellular and Infection Microbiology

Genome-wide bioinformatic prediction and experimental evaluation of potential RNA thermometers. Regulation of heat-shock genes in bacteria: Differential control of Salmonella heat shock operons by structured mRNAs. It is easily conceivable that temperature-responsive structures such as RNATs can be discovered by this technology and a first step in this direction has been undertaken by probing the whole rrna transcriptome at different temperatures Wan et amd.

Role yhermometers cis-acting RNA sequences in the temperature-dependent expression of the multiadhesive lig proteins in Leptospira interrogans. Quite surprisingly, no prokaryotic RNA structurome has been reported so far. In vivo genome-wide profiling thwrmometers RNA secondary structure reveals novel regulatory features. Plant Cell 24, — The melting temperature of each sequenced transcript was measured at a single nucleotide resolution, which led to the identification of RNA regions that undergo conformational changes in a physiological range of temperature.


Mfold web server for nucleic acid folding and hybridization prediction. Liberation of the RBS permits formation of the translation initiation complex and translation occurs. Two identical RNATs were found upstream of the Leptospira interrogans virulence genes ligA and ligB coding for putative lipoproteins important for adhesion and complement thermkmeters Matsunaga et al.

These regions might have a role in temperature-mediated post-transcriptional gene regulation. In this article, we briefly recapitulate how RNATs have been discovered in the past, before we go on to discuss the potential of recently established next-generation sequencing techniques for genome-wide identification of new regulatory RNA elements.

Transient RNA structure features are evolutionarily conserved and can be computationally predicted. Another important aspect is that the folding process of a nascent bacterial RNA is coupled to its transcription and translation.

Since then numerous RNAT-controlled heat shock and virulence genes have been discovered. Long distance interactions allow formation of tertiary zippeers, like pseudoknots or kissing loops.

switchfs The most common genes subjected to RNAT-mediated regulation code for small heat shock proteins sHSPsATP-independent chaperones that maintain client proteins in a folding-competent state.