/ Fractogel® EMD SO3 (S), (M). -. Ion Exchange chromatography using strong cation exchangers. Fractogel® ion exchangers are cross-linked. Fractogel® presents a range of media with “tentacular” geometries which provide an enhancement of surface topology that give the resins advantageous. Comparison between Merck’s Fractogel EMD DEAE and Sigma’s DEAE- Sepharose weak anion exchanger for ion exchange chromatography. I am purifying an.
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Characterisation of a novel stationary phase designed for production-scale purification mabs [IF: Daniel Magiera 2 Estimated H-index: Montelaro Louisiana State University. Elution of lipoprotein fractions containing apolipoproteins E and A-I in size exclusion on Superose 6 columns fractogeel sensitive to mobile phase pH and ionic strength. Dependence of optimal packing velocity on column size.
Earle Stellwagen 33 Estimated H-index: For lysozyme and aprotinin with pI above experimental pH, dependence of pH on retention was generally low, though some pronounced decrease of retention with increasing pH was observed for CM HyperZ. One main advantage of SEC is that: Unger University of MainzM. Are you looking for Thus, gels made for SEC should have controlled range of size of the pores.
New ion exchangers for the chromatography of biopolymers. The most fractohel and widely distributed packings consist of glycidyl methacrylate copolymers, agarose, or composite gels containing dextran. dractogel
EMD Millipore Ion Exchange Chromatography Media, Fractogel®
Resins with good pure aprotinin capacity also performed well for aprotinin in fermentation broth, but the highest relative capacity was obtained with MacroPrep High S having a fairly low pure component dynamic capacity. View in Source Cite this paper. Cation-exchange chromatography of monoclonal antibodies: Publisher Summary Size exclusion chromatography SEC is the predominant chromatographic technique for determining the molecular weight distribution of macromolecules. Particle size distribution was determined by two independent methods, coulter counting and SEM pictures, with fair agreement.
Binding strength data of cation-exchange resins as a function of ionic strength depends on the protein, but binding and elution at high salt concentration may in general be performed with MacroPrep resins.
The effect of competitive binding from yeast fermentation components on dynamic binding capacity of aprotinin was studied showing a significant decrease in binding capacity. Testing was done with three proteins: Packing of Toyopearl columns for gel filtration: Influence of surface modification on protein retention in frcatogel chromatography: Packing of toyopearl column for gel filtration: Chun-hua Cai 2 Estimated H-index: Determination of alkaline vractogel aggregation by size exclusion high-performance liquid chromatography with low-angle laser light scattering detection.
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John Westerlund 1 Estimated H-index: Size-exclusion chromatography of plasma proteins with high molecular masses. Chromatography Methacrylate Molar mass distribution Organic chemistry Ionic strength Materials science Size-exclusion chromatography Macromolecule Polymer Agarose Glycidyl methacrylate. Other Papers By First Author. Influence of packing velocity on column performance. fracttogel
Marina UrmannHeiner Graalfs. Chatziathanassiou University of Mainz.
Jerker Porath 49 Estimated H-index: The separation mode is based on the differential penetration of molecules of different size and shape into the pores of the stationary phase. Strong and weak cation-exchangers were compared for a number of chromatographic parameters, i.
Yoshio Kato 19 Estimated H-index: Djuro Josic 27 Estimated H-index: Results of this paper may be used in the selection of resins for further testing in biopharmaceutical protein purification process development. Evaluation using different retention models journal of chromatography a [IF: The methacrylate-based copolymer Fractogel EMD BioSEC used for large-scale purification of biological gractogel is introduced to overcome the drawback derived from frxctogel poor pressure stability of soft gels.
Efficiency results present the expected trend of increasing dependence of plate height as a function of increasing flow rate, and the highest flow dependence was observed for Fractogel EMD COO.